Coding
Part:BBa_K1539003:Design
Designed by: Coleen Tran Group: iGEM14_GeorgiaTech (2014-10-09)
Beta Subunit_sMMO A
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The gene had to be codon optimized for E.coli, with originating gene coming from Methylococcus capsulatus
Source
Gene ordered from Genscript in pUC57_Kan backbone